Atalanta's Belgian defender Timothy Castagne (left) and Lazio's Serbian midfielder Sergej Milinkovic-Savic go for a header during the Italian Serie A football match Atalanta vs Lazio played on June 24, 2020 behind closed doors at the Atleti Azzurri d'Italia stadium in Bergamo. (MIGUEL MEDINA / AFP)

ROME - Lazio's chase for the Serie A title was dealt a blow on Wednesday as they suffered a 3-2 away defeat to Atalanta.

The defeat, coupled with Juventus's victory on Monday, extended Lazio's gap to the Bianconeri to four points

The Biancocelesti had created an all-time club record with a 21-game unbeaten run to sit just one point behind leaders Juventus before the 27th round of matches.

Lazio surged into a 2-0 lead after just 11 minutes thanks to an own goal from Marten de Roon and a rocket from Sergej Milinkovic-Savic.

However, Atalanta, who have scored the most goals in the current Serie A season, fought back later on, as Robin Gosens, Ruslan Malinovskyi and Jose Luis Palomino scored to help the home side claim a strong comeback win.

The defeat, coupled with Juventus's victory on Monday, extended Lazio's gap to the Bianconeri to four points.

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Inter Milan's Cristiano Biraghi scores his side's second goal during the Serie A soccer match between Inter Milan and Sassuolo at the San Siro Stadium, in Milan, Italy, June 24, 2020. (LUCA BRUNO / AP)

Third-placed Inter Milan's Scudetto hopes were also dealt a blow after they were held to a 3-3 draw with Sassuolo.

With the two sides deadlocked at 2-2 after 85 minutes, Borja Valero's tap-in at the back post helped the Nerazzurri restore their lead, only for Giangiacomo Magnani to equalize three minutes later.

Inter Milan ended the match with ten men as defender Milan Skriniar collected a second yellow card in the dying minutes.

Inter now trail Juventus by eight points.

In the day's other fixture, Edin Dzeko bagged a brace, helping Roma come from behind to beat Sampdoria 2-1.